Review



10xuas fragment  (Addgene inc)


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    Structured Review

    Addgene inc 10xuas fragment
    10xuas Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10xuas+fragment/pJFRC81-10XUAS-IVS-Syn21-GFP-p10+(Plasmid+%2336432)/pmc13030971-294-18-23
    Average 93 stars, based on 67 article reviews
    10xuas fragment - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: A genetic strategy for targeting local astrocytes in adult Drosophila.
    Article Snippet: The 530 bp fragment of the part of the nlg-synthetic_Notch-anti_CD19 that was cut away from the backbone was amplified from nSyb-nlg-SNTG4-V5 and assembled with the amplified QF2 transcription factor with overlapping homology between inserts and backbone by Gibson assembly (NEB). .. 10xUAS-IVS-nSyb-CD19 The 13xLexAop2 fragment of the construct 13xLexAop2-IVS-nsybCD19 (gift from Carlos Lois, Caltech) was removed and replaced by the 10xUAS fragment isolated from pJFRC81-10xUAS-IVS-Syn21-GFP-P10 (Addgene #36432) with Bgl2 and Hind3 by conventional T4 DNA ligase-mediated cloning (NEB). ..

    Article Title: A genetic strategy for targeting local astrocytes in adult Drosophila
    Article Snippet: The 530 bp fragment of the part of the nlg-synthetic_Notch-anti_CD19 that was cut away from the backbone was amplified from nSyb-nlg-SNTG4-V5 and assembled with the amplified QF2 transcription factor with overlapping homology between inserts and backbone by Gibson assembly (NEB). .. The 13xLexAop2 fragment of the construct 13xLexAop2-IVS-nsybCD19 (gift from Carlos Lois, Caltech) was removed and replaced by the 10xUAS fragment isolated from pJFRC81-10xUAS-IVS-Syn21-GFP-P10 (Addgene #36432) with Bgl2 and Hind3 by conventional T4 DNA ligase-mediated cloning (NEB). ..

    Article Title: Genetic targeting of astrocytes associated with specific neuronal circuit in adult Drosophila
    Article Snippet: .. The 13xLexAop2 fragment of the construct 13xLexAop2-IVS-nsyb::CD19 (gift from Carlos Lois, Caltech) was removed and replaced by the 10xUAS fragment isolated from pJFRC81-10xUAS-IVS-Syn21-GFP-P10 (Addgene #36432) with Bgl2 and Hind3 by conventional T4 DNA ligase-mediated cloning (NEB). ..

    Isolation:

    Article Title: A genetic strategy for targeting local astrocytes in adult Drosophila.
    Article Snippet: The 530 bp fragment of the part of the nlg-synthetic_Notch-anti_CD19 that was cut away from the backbone was amplified from nSyb-nlg-SNTG4-V5 and assembled with the amplified QF2 transcription factor with overlapping homology between inserts and backbone by Gibson assembly (NEB). .. 10xUAS-IVS-nSyb-CD19 The 13xLexAop2 fragment of the construct 13xLexAop2-IVS-nsybCD19 (gift from Carlos Lois, Caltech) was removed and replaced by the 10xUAS fragment isolated from pJFRC81-10xUAS-IVS-Syn21-GFP-P10 (Addgene #36432) with Bgl2 and Hind3 by conventional T4 DNA ligase-mediated cloning (NEB). ..

    Article Title: A genetic strategy for targeting local astrocytes in adult Drosophila
    Article Snippet: The 530 bp fragment of the part of the nlg-synthetic_Notch-anti_CD19 that was cut away from the backbone was amplified from nSyb-nlg-SNTG4-V5 and assembled with the amplified QF2 transcription factor with overlapping homology between inserts and backbone by Gibson assembly (NEB). .. The 13xLexAop2 fragment of the construct 13xLexAop2-IVS-nsybCD19 (gift from Carlos Lois, Caltech) was removed and replaced by the 10xUAS fragment isolated from pJFRC81-10xUAS-IVS-Syn21-GFP-P10 (Addgene #36432) with Bgl2 and Hind3 by conventional T4 DNA ligase-mediated cloning (NEB). ..

    Article Title: Genetic targeting of astrocytes associated with specific neuronal circuit in adult Drosophila
    Article Snippet: .. The 13xLexAop2 fragment of the construct 13xLexAop2-IVS-nsyb::CD19 (gift from Carlos Lois, Caltech) was removed and replaced by the 10xUAS fragment isolated from pJFRC81-10xUAS-IVS-Syn21-GFP-P10 (Addgene #36432) with Bgl2 and Hind3 by conventional T4 DNA ligase-mediated cloning (NEB). ..

    Cloning:

    Article Title: A genetic strategy for targeting local astrocytes in adult Drosophila.
    Article Snippet: The 530 bp fragment of the part of the nlg-synthetic_Notch-anti_CD19 that was cut away from the backbone was amplified from nSyb-nlg-SNTG4-V5 and assembled with the amplified QF2 transcription factor with overlapping homology between inserts and backbone by Gibson assembly (NEB). .. 10xUAS-IVS-nSyb-CD19 The 13xLexAop2 fragment of the construct 13xLexAop2-IVS-nsybCD19 (gift from Carlos Lois, Caltech) was removed and replaced by the 10xUAS fragment isolated from pJFRC81-10xUAS-IVS-Syn21-GFP-P10 (Addgene #36432) with Bgl2 and Hind3 by conventional T4 DNA ligase-mediated cloning (NEB). ..

    Article Title: A genetic strategy for targeting local astrocytes in adult Drosophila
    Article Snippet: The 530 bp fragment of the part of the nlg-synthetic_Notch-anti_CD19 that was cut away from the backbone was amplified from nSyb-nlg-SNTG4-V5 and assembled with the amplified QF2 transcription factor with overlapping homology between inserts and backbone by Gibson assembly (NEB). .. The 13xLexAop2 fragment of the construct 13xLexAop2-IVS-nsybCD19 (gift from Carlos Lois, Caltech) was removed and replaced by the 10xUAS fragment isolated from pJFRC81-10xUAS-IVS-Syn21-GFP-P10 (Addgene #36432) with Bgl2 and Hind3 by conventional T4 DNA ligase-mediated cloning (NEB). ..

    Article Title: Genetic targeting of astrocytes associated with specific neuronal circuit in adult Drosophila
    Article Snippet: .. The 13xLexAop2 fragment of the construct 13xLexAop2-IVS-nsyb::CD19 (gift from Carlos Lois, Caltech) was removed and replaced by the 10xUAS fragment isolated from pJFRC81-10xUAS-IVS-Syn21-GFP-P10 (Addgene #36432) with Bgl2 and Hind3 by conventional T4 DNA ligase-mediated cloning (NEB). ..



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